Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7
cell line
MCF7
cell type
ER+ breast cancer cell line
genotype
sgNT
chip antibody
ER (Millipore, #06-935)

Sequenced DNA Library

library_name
GSM6716975
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were fixed with 1% formaldehyde for 10 minutes. After quenching, chromatin was prepared using SimpleChIP Enzymatic Chromatin Kit from cell signaling. ChIP-seq libraries were prepared using the KAPA HTP Library Preparation Kit.

Sequencing Platform

instrument_model
Illumina NovaSeq 6000

hg38

Number of total reads
58015053
Reads aligned (%)
98.5
Duplicates removed (%)
26.8
Number of peaks
2593 (qval < 1E-05)

hg19

Number of total reads
58015053
Reads aligned (%)
97.6
Duplicates removed (%)
27.4
Number of peaks
1762 (qval < 1E-05)

Base call quality data from DBCLS SRA